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Lipids, proteins and corneocyte adhesion.

S J Chapman1, A Walsh, S M Jackson

  • 1Department of Dermatology, Royal Liverpool Hospital, UK.

Archives of Dermatological Research
|January 1, 1991
PubMed
Summary
This summary is machine-generated.

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Corneosomes, not lipids, are the main factor in skin cell (corneocyte) cohesion. While extracellular lipids play a minor role, corneosomes are crucial for maintaining skin barrier integrity.

Area of Science:

  • Dermatology
  • Skin Biology
  • Biophysics

Background:

  • Corneocyte cohesion is vital for skin barrier function.
  • The relative contributions of extracellular lipids, corneosomes, and lipid envelopes to cohesion are not fully understood.

Purpose of the Study:

  • To determine the primary factor responsible for corneocyte cohesion in pig ear stratum corneum.
  • To elucidate the roles of membrane-coating granule (MCG) lipids, corneosomes, and covalently bound lipid envelopes.

Main Methods:

  • Direct measurement of stratum corneum cohesion using cohesometry.
  • Alteration of cohesion by solvent extraction of MCG lipids.
  • Ultrastructural analysis and trypsin digestion to assess corneosome involvement.

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Main Results:

  • Potent solvent extraction of MCG lipids correlated with increased cohesion, attributed to fused lipid envelopes.
  • MCG lipids appear to have an anti-cohesive role, preventing lipid envelope fusion.
  • Corneosome numbers and cohesion increased from stratum corneum surface to granular layer.
  • Trypsin digestion of corneosomes induced superficial cell shedding.

Conclusions:

  • Corneosomes are the major determinant of stratum corneum cohesion.
  • MCG lipids play a minor cohesive role, primarily supporting barrier function.
  • Covalently bound lipid envelopes are unlikely to mediate normal stratum corneum cohesion.