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Related Experiment Video

Updated: Jul 3, 2026

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Published on: October 5, 2016

New developments in low-dose insemination technology.

J M Vazquez¹, J Roca, M A Gil

¹Department of Animal Medicine and Surgery, Campus de Espinardo, University of Murcia, E-30071 Murcia, Spain. vazquez@um.es

|August 5, 2008

Summary

This summary is machine-generated.

New swine insemination techniques reduce sperm count needs for pregnancy. Post-cervical and deep intrauterine insemination (DUI) improve efficiency with fresh, frozen, or sex-sorted semen.

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Last Updated: Jul 3, 2026

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

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Published on: October 5, 2016

Minimally Invasive Embryo Transfer and Embryo Vitrification at the Optimal Embryo Stage in Rabbit Model

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Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Published on: July 7, 2023

Area of Science:

Veterinary Science
Animal Reproduction

Background:

Traditional artificial insemination (AI) in swine requires significant sperm numbers.
Advances in reproductive technologies necessitate efficient sperm utilization methods.

Purpose of the Study:

To evaluate novel nonsurgical and laparoscopic insemination procedures in swine.
To assess the efficacy of reduced sperm numbers with these new techniques.

Main Methods:

Post-cervical insemination into the uterine body.
Deep intrauterine insemination (DUI) directly into the uterine horn.
Laparoscopic insemination into the oviduct.

Main Results:

Post-cervical insemination achieved pregnancy with a threefold reduction in fresh sperm.
DUI enabled up to 20-fold reduction in fresh sperm or sixfold in frozen/thawed sperm, maintaining high fertility.
Laparoscopic techniques are effective with diluted and sex-sorted spermatozoa.

Conclusions:

Nonsurgical and laparoscopic insemination procedures significantly reduce sperm requirements in swine.
These advanced techniques facilitate high fertility rates with various semen types, including cooled, frozen-thawed, and sex-sorted options.
Optimized insemination strategies enhance the application of current sperm technologies in swine breeding.