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Updated: Jul 3, 2026

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Published on: September 11, 2020

Phage-associated mutator phenotype in group A streptococcus.

Julie Scott¹, Prestina Thompson-Mayberry, Stephanie Lahmamsi

¹Department of Pharmaceutical Sciences, The University of Oklahoma College of Pharmacy, P.O. Box 26901, Oklahoma City, OK 73190, USA.

Journal of Bacteriology

|August 5, 2008

Summary

A novel bacteriophage in Streptococcus pyogenes controls DNA repair, causing a mutator phenotype. This dynamic system allows bacteria to adapt to changing environments by altering mutation rates.

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Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

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Published on: September 11, 2020

Purification of a High Molecular Mass Protein in Streptococcus mutans

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Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

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Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Defects in DNA mismatch repair (MMR) are common in bacteria, leading to a mutator phenotype.
Streptococcus pyogenes exhibits dynamic control over its MMR system.

Purpose of the Study:

To identify the genetic element controlling MMR in S. pyogenes SF370.
To elucidate the mechanism of dynamic MMR regulation by a prophage.

Main Methods:

Genetic analysis of Streptococcus pyogenes strain SF370.
Prophage identification, characterization, and analysis of its integration site.
Gene expression analysis of mutS and mutL.

Main Results:

A unique prophage, SF370.4, integrates within the mutL gene, blocking its expression and causing a mutator phenotype.
Prophage excision during rapid growth allows mutL expression, restoring functional MMR.
MMR is functional in exponentially growing cells but defective in stationary phase.

Conclusions:

Bacteriophage-mediated dynamic control of MMR is a novel mechanism for bacterial adaptation.
This system allows S. pyogenes to modulate mutation rates for environmental response.
Prophage-mediated MMR regulation may be widespread in S. pyogenes populations.