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Updated: Jul 3, 2026
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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
[Gastric lymphoma. Morphological and immunohistochemical study].
D Ferariu1, M Danciu, Delia Ciobanu
1Spitalul Clinic de Urgenţă Sf. Spiridon Iaşi Serviciul de Anatomie Patologică şi Prosectură.
Summary
Gastric lymphomas, primarily B-cell types, were analyzed using immunohistochemistry. This method aids in identifying lymphoepithelial lesions and Helicobacter pylori, crucial for accurate diagnosis and classification.
Area of Science:
- Gastroenterology
- Oncology
- Pathology
Background:
- Extranodal lymphomas frequently occur in the gastrointestinal tract, with gastric lymphoma being the most common.
- Helicobacter pylori infection plays a significant role in the pathogenesis of gastric lymphoma, influencing treatment strategies.
Purpose of the Study:
- To describe the morphological and immunohistochemical features of gastric lymphomas.
- To evaluate the utility of immunohistochemistry in diagnosing gastric lymphomas and identifying associated factors.
Main Methods:
- Thirty-four gastric lymphomas were examined using routine histopathology and immunohistochemical staining.
- Antibodies used included markers for B-cells (CD20, CD79a), T-cells (CD3, CD5), lymphoma subtypes (CD23, CD30, BCL2, BCL6, ALK1), proliferation (Ki67), epithelial markers (CK-cocktail), and H. pylori.
Main Results:
- All lymphomas were located in the gastric antrum, predominantly as solitary, large tumors.
- 97% were B-cell lymphomas, including 41.17% mucosa-associated lymphatic tissue (MALT) lymphomas. One peripheral T-cell lymphoma was identified.
- Immunohistochemistry enhanced detection of lymphoepithelial lesions specific to MALT lymphomas and increased H. pylori detection sensitivity by 22%.
Conclusions:
- Immunohistochemistry is essential for diagnosing gastric lymphomas.
- It aids in identifying lymphoepithelial lesions and detecting H. pylori infection.
- This technique is mandatory for classifying lymphomas according to WHO criteria.
