Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Designing Growth Media for Bioreactors

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...

Microbial Growth Media

Microbial Growth Media

Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.

Microbial Growth Measurement: Indirect Methods

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...

Methods for Controlling Microbial Growth

Methods for Controlling Microbial Growth

Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...

Microbial Growth Measurement: Direct Methods

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Group B streptococcal membrane vesicles induce proinflammatory responses in neonatal meninges.

Infection and immunity·2026

Same author

Identification of DksA as a novel pro-inflammatory mediator of <i>Pseudomonas aeruginosa</i> under conditions mimicking chronic cystic fibrosis lung infection.

Virulence·2026

Same author

Biosynthesis of glucosaminyl phosphatidylglycerol in <i>Pseudomonas aeruginosa</i>.

Journal of bacteriology·2025

Same author

A call for the United States to continue investing in science.

ASM case reports·2025

Same author

Genome sequences of fifteen soil bacteria isolated from the Dallas-Fort Worth Area, United States.

Microbiology resource announcements·2025

Same author

Analysis of Teg41 and PSMα promoter activity using a divergent fluorescent reporter plasmid.

mSphere·2025

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

Revisiting the host as a growth medium.

Stacie A Brown¹, Kelli L Palmer, Marvin Whiteley

¹Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas 78712, USA.

Nature Reviews. Microbiology

|August 6, 2008

Summary

This summary is machine-generated.

Understanding how bacterial pathogens use nutrients inside the body is key to fighting infections. Targeting carbon metabolism in bacteria offers a promising new strategy for developing effective antibiotics.

More Related Videos

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Published on: July 4, 2018

Related Experiment Videos

Last Updated: Jul 3, 2026

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Published on: July 4, 2018

Area of Science:

Microbiology
Infectious Diseases
Metabolic Pathways

Background:

Bacterial pathogenesis has been studied for over 200 years.
Understanding pathogen nutrient acquisition in vivo is crucial but limited.
In vivo physiology and metabolism of human pathogens remain poorly understood.

Purpose of the Study:

To review the impact of in vivo carbon sources on disease.
To highlight carbon metabolism as a target for novel antibiotic development.

Main Methods:

Literature review of studies on bacterial pathogenesis and metabolism.
Analysis of the role of carbon sources in microbial growth and survival within the host.
Exploration of metabolic pathways as potential antimicrobial targets.

Main Results:

In vivo carbon sources significantly influence bacterial growth and disease progression.
Specific metabolic pathways are critical for pathogen survival.
Targeting these pathways presents a viable strategy for combating bacterial infections.

Conclusions:

Carbon metabolism is a critical determinant of bacterial pathogenesis.
Exploiting the unique metabolic requirements of pathogens can lead to new antibiotic therapies.
Further research into in vivo metabolic strategies is essential for developing effective treatments against infectious diseases.