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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

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Published on: May 29, 2018

Sensitive molecular binding assay using a photonic crystal structure in total internal reflection.

Yunbo Guo1, Charles Divin, Andrzej Myc

  • 1Center for Ultrafast Optical Science, University of Michigan, Ann Arbor, MI 48109-2099, USA.

Optics Express
|August 6, 2008
PubMed
Summary

This study introduces a novel optical sensor for label-free biomolecular detection. The photonic crystal sensor achieves highly sensitive, real-time measurements of molecular binding with improved detection limits compared to existing technologies.

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Area of Science:

  • Photonics
  • Biomolecular Sensing
  • Optical Physics

Background:

  • Label-free biosensing is crucial for real-time monitoring of molecular interactions.
  • Existing methods like surface plasmon resonance (SPR) have limitations in sensitivity and configuration.
  • Photonic crystals offer unique optical properties for sensing applications.

Purpose of the Study:

  • To propose and demonstrate a novel optical sensor for label-free biomolecular binding assays.
  • To utilize a one-dimensional photonic crystal in a total-internal-reflection geometry for enhanced sensitivity.
  • To enable real-time measurements of binding dynamics with an open interface.

Main Methods:

  • Fabrication and characterization of a one-dimensional photonic crystal.
  • Implementation of a total-internal-reflection geometry for optical measurements.
  • Differential reflectance measurements to detect spectral shifts and intensity changes.

Main Results:

  • Demonstrated a simple, open-interface optical sensor configuration.
  • Achieved a narrow optical resonance for sensitive molecular binding detection.
  • Obtained a detection limit of 6 x 10(-5) nm for molecular layer thickness.
  • Reported a refractive index resolution of 3 x 10(-8) RIU.
  • Showcased significant improvements over state-of-the-art SPR systems.

Conclusions:

  • The proposed one-dimensional photonic crystal sensor offers a highly sensitive and real-time platform for label-free biomolecular binding assays.
  • This novel sensor design represents a significant advancement in biosensing technology.
  • The system's performance surpasses current SPR-based methods, paving the way for new diagnostic and research tools.