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Algorithm and simulation for analysis of bio-images obtained by aperture diffraction based optical MEMS.

Xiaodong Zhou¹, Daniel Puiu Poenar, Kai Yu Liu

¹Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602. donna-zhou@imre.a-star.edu.sg

|August 6, 2008

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This study introduces a new optical diffraction method for detecting transparent living cells in microfluidic devices. The technique analyzes diffraction patterns to determine cell size, refractive index, or position.

Area of Science:

Optical Physics
Biotechnology
Microfluidics

Background:

Detecting transparent living cells in microfluidic devices presents challenges due to their low contrast.
Existing methods may require staining or complex imaging setups.

Purpose of the Study:

To develop a novel optical diffraction-based method for label-free detection of transparent living cells.
To enable extraction of cell parameters like size, refractive index, and position.

Main Methods:

Utilizing optical diffraction from apertures or aperture arrays to generate far-field diffraction patterns.
Developing analysis software to interpret diffraction patterns for cell parameter extraction.
Simulating and experimentally verifying the method using transparent microparticles and fabricated apertures.

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Main Results:

Demonstrated the ability to extract cell parameters (size, refractive index, position) from diffraction patterns.
Validated simulation results with experimental diffraction patterns of microparticles.
Successfully detected transparent cells in a microfluidic chamber.

Conclusions:

The proposed optical diffraction method offers a promising approach for label-free detection and characterization of transparent living cells.
This technique has potential applications in microfluidic cell analysis and diagnostics.