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Related Experiment Video

Updated: Jul 3, 2026

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

RNA expression analysis from formalin fixed paraffin embedded tissues.

Susan M Farragher1, Austin Tanney, Richard D Kennedy

  • 1Almac Diagnostics, Craigavon, 19 Seagoe Industrial Estate, Armagh BT635QD, UK.

Histochemistry and Cell Biology
|August 6, 2008
PubMed
Summary
This summary is machine-generated.

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Formalin fixation and paraffin embedding (FFPE) preserves tissues but damages nucleic acids. Recovering this genetic data is crucial for personalized medicine advancements using techniques like RT-PCR and microarrays.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Formalin fixation and paraffin embedding (FFPE) is a standard global technique for tissue preservation.
  • FFPE preserves tissue morphology but significantly degrades nucleic acids (DNA and RNA).
  • Clinical adoption of gene expression analysis necessitates accessing FFPE-archived genetic information.

Purpose of the Study:

  • To review advancements in nucleic acid analysis from FFPE tissues.
  • To explore the potential of FFPE-derived data for personalized medicine.
  • To highlight opportunities for leveraging FFPE archives in clinical practice.

Main Methods:

  • Literature review of techniques for nucleic acid extraction and analysis from FFPE samples.
  • Discussion of gene expression profiling methods (e.g., RT-PCR, microarrays).

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miRNA Expression Analyses in Prostate Cancer Clinical Tissues

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Last Updated: Jul 3, 2026

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Published on: September 2, 2013

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

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  • Analysis of challenges and opportunities in FFPE molecular diagnostics.

    • Main Results:

    • Progress has been made in overcoming nucleic acid damage caused by FFPE.
    • Techniques allow for gene expression profiling from FFPE tissues.
    • FFPE archives represent a valuable resource for retrospective studies.

    Conclusions:

    • Despite FFPE-induced nucleic acid damage, significant progress enables valuable molecular analysis.
    • Analyzing FFPE samples offers unique opportunities for personalized medicine development.
    • Further research can unlock the full potential of FFPE archives for clinical applications.