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Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
Published on: March 11, 2020
Genome sequence and characterization of a new virus infecting Mikania micrantha H.B.K
Rui-Long Wang1, Ling-Wen Ding, Qiao-Yang Sun
1State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, East Campus, Bei San Road, 510006 Guangzhou, Guangdong, People's Republic of China.
Abstract:
The complete RNA genomic sequence of a new virus infecting Mikania micrantha, designated as Mikania micrantha wilt virus (MMWV), has been determined. The genomic sequence and the predicted gene products of MMWV were similar to those of the other viruses of the genus Fabavirus. The MMWV nucleotide sequence showed 75.6% identity to that of gentian mosaic virus, 56.6 and 57% identity to those of two Broad bean wilt virus 1 isolates, and between 55.7 and 58% identity to those of seven Broad bean wilt virus 2 isolates. Our results suggested that MMWV represents a distinct isolate of the candidate species Gentian mosaic virus.
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