Isolation and characterisation of cytopathic avian enteroviruses from broiler chicks

Cytopathic viruses were isolated from the faeces, pancreas and caecal contents of broiler chicks in two cases of a stunting syndrome. Two representative strains, designated AAF7 and M-8, were identified as enteroviruses on the basis of their size (25 nm in diameter), RNA in the viral core, virus growth in the cytoplasm, resistance to chloroform, trypsin and acid, and partial heat-stabilisation to molar magnesium chloride. They formed irregular plaques in chick kidney cell cultures. Numerous granula eosinophilic inclusions were induced in the cytoplasm of chicken kidney cell cultures infected with the viruses. Both isolates reacted partially with the G4260 strain of avian nephritis virus but not with the VR strain of avian encephalomyelitis virus.