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An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Published on: October 4, 2018
Comparative studies on the pathogenicity of cytopathic avian enteroviruses (avian nephritis virus) isolated from
K Takase1, T Uchimura, M Yamamoto
1Chemo-Sero-Therapeutic Research Institute, Shimizu-cho, Kumamoto, Japan.
Abstract:
The pathogenicities of three cytopathic avian enteroviruses, strains AAF7, M-8, and G-4260 which were classified as avian nephritis viruses, were compared following inoculation into chicken kidney cell (CKC) cultures, embryonating eggs, and 1-day-old chicks. Strain M-8 produced the largest plaques, highest yields in CKC cultures, highest pathogenicity for embryonating eggs, and severest lesions in the kidney of experimentally-infected chicks. In contrast, strain AAF7 was the least pathogenic. All strains caused growth retardation and nephritis in experimentally-infected chicks.
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