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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

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Aptamer switch probe based on intramolecular displacement.

Zhiwen Tang¹, Prabodhika Mallikaratchy, Ronghua Yang

¹Center for Research at the Bio/Nano Interface, Department of Chemistry, Shands Cancer Center and UF Genetics Institute, University of Florida, Gainesville, Florida 32611-7200, USA.

Journal of the American Chemical Society

|August 6, 2008

Summary

This summary is machine-generated.

A new aptamer switch probe (ASP) uses intramolecular signal transduction for sensitive molecular detection. This aptamer-based molecular probe design offers enhanced fluorescence for target identification, showing great potential for bioimaging applications.

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Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Aptamer-based probes are crucial for molecular detection.
Intramolecular signal transduction offers a sensitive detection mechanism.

Purpose of the Study:

To demonstrate a novel aptamer-based molecular probe design utilizing intramolecular signal transduction.
To engineer and validate an aptamer switch probe (ASP) for sensitive and selective target detection.

Main Methods:

Designed an aptamer switch probe (ASP) with an aptamer, cDNA, PEG linker, fluorophore, and quencher.
Engineered ASPs for ATP and human alpha-thrombin targets.
Investigated fluorescence changes upon target binding through intramolecular signal transduction.

Main Results:

The ASP design showed fluorescence enhancement upon target molecule binding.
Demonstrated quantitative fluorescence changes mediated by varying target concentrations.
Achieved excellent selectivity and prompt response for both ATP and human alpha-thrombin targets.

Conclusions:

The aptamer switch probe (ASP) design enables efficient intramolecular signal transduction for molecular detection.
ASPs exhibit promising potential for applications in biochips and in situ imaging due to their sensitivity, stability, and reusability.