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Colony-stimulating factor-1 blocks early pregnancy in mice
B Tartakovsky1, O Goldstein, N Brosh
1Department of Chemical Immunology, Weizmann Institute of Science, Rehovot, Israel.
Biology of Reproduction
|May 1, 1991
Summary
Immune-related pregnancy failures in mice were studied. Colony-stimulating factor-1 (CSF-1) was identified as a key factor causing pregnancy block and embryo resorption in specific mating combinations.
Area of Science:
- Reproductive Immunology
- Developmental Biology
- Immunology
Background:
- Immune-related pregnancy failures are a concern in humans.
- Experimental models are needed to understand these failures.
- Specific mating combinations can lead to pregnancy complications.
Purpose of the Study:
- To elucidate mechanisms of immune-related pregnancy failures.
- To establish a mouse model for studying pregnancy block and abortion.
- To identify factors responsible for male-specific pregnancy failures.
Main Methods:
- Pre-immunization of female mice with syngeneic regressor tumor.
- Mating females with different male strains (B6D2F-1, DBA/2, CBA/J, C57BL/6).
- Injection of colony-stimulating factor-1 (CSF-1) during early pregnancy.
Main Results:
- Pre-immunized females showed male-specific pregnancy failures.
- Colony-stimulating factor-1 (CSF-1) was identified as responsible for pregnancy block and embryo resorption.
- Low-dose CSF-1 injection blocked gestation in sensitive matings and caused fetal resorptions.
Conclusions:
- A novel mechanism for pregnancy failure involving cytokines and early embryonic development is suggested.
- CSF-1 plays a critical role in specific mating-induced pregnancy failures.
- Cytokine-mediated effects on early embryonic development can lead to pregnancy loss.