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Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

A quick method to estimate the T-DNA copy number in transgenic plants at an early stage after transformation, using

M P Does1, B M Dekker, M J de Groot

  • 1MOGEN International nv, Leiden, Netherlands.

Plant Molecular Biology
|July 1, 1991
PubMed

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...