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Related Experiment Video

Updated: Jul 3, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microfluidic single-cell analysis of intracellular compounds.

Tzu-Chiao Chao1, Alexandra Ros

  • 1Department of Chemistry and Biochemistry, Arizona State University, Box 871604, Tempe, AZ 85287-1604, USA.

Journal of the Royal Society, Interface
|August 7, 2008
PubMed
Summary

Single-cell analysis using microfluidic devices overcomes limitations of traditional cell ensemble studies. These advanced methods offer higher sensitivity for analyzing individual cell responses and biological insights.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Traditional biological analyses average responses from large cell populations (10^3-10^6 cells).
  • This averaging obscures critical individual cell variations in proliferation, stimuli response, and disease onset.
  • There is a growing need for single-cell analytical methods to gain deeper biological insights.

Purpose of the Study:

  • To review advancements in microfluidic single-cell analysis of intracellular compounds.
  • To highlight the transition from proof-of-principle devices to biologically relevant applications.
  • To emphasize the advantages of microfluidics for sensitive single-cell measurements.

Main Methods:

  • Microfluidic or lab-on-a-chip devices are employed for single-cell analysis.

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Single-cell Microfluidic Analysis of Bacillus subtilis
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Single-cell Microfluidic Analysis of Bacillus subtilis

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Last Updated: Jul 3, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
09:43

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Published on: March 8, 2024

Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

  • These devices integrate essential steps: cell selection, navigation, positioning, lysis, separation, and analyte detection.
  • Compartmentalization in microfluidic devices minimizes dilution and enhances detection sensitivity.

    • Main Results:

    • Microfluidic devices enable precise manipulation and analysis of individual cells.
    • They offer integrated solutions for various single-cell analysis processes.
    • These systems significantly improve detection sensitivity for intracellular compounds.

    Conclusions:

    • Microfluidic technology is pivotal for advancing single-cell analysis.
    • These methods provide crucial insights into genomics, proteomics, transcriptomics, and systems biology.
    • The review showcases the significant progress and biological relevance of microfluidic single-cell analysis.