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Updated: Jul 3, 2026
Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
[Identification of a novel allele human leukocyte antigen B*4609]
Yang Chen1, Jian-ping Li, Kun-lian Zhang
1Liaoning Blood Center, Shenyang, Liaoning, 110044 P. R. China. ljp_63@yahoo.com.
Objective:
To identify a novel human leukocyte antigen (HLA) allele.
Methods:
HLA typing was carried out with PCR-SSOP. Molecular cloning and DNA sequencing were used to identify the sequence of a potential novel allele and the difference between this new allele and other known alleles was analyzed.
Results:
HLA genotyping of one sample gave different results. The sequencing results showed that the HLA B alleles of the proband were B*151101 and a novel allele. The nucleotide sequence of the novel allele was different from all other known B alleles. It had one nucleotide change from the closest matching allele B*460101 at nucleotide 527 (A to T) in exon 3, resulting in an amino acid change from E (GAG) to V (GTG) at codon 176.
Conclusion:
A novel HLA B allele was identified and officially designated as HLA B*4609 by WHO Nomenclature Committee for Factors of the HLA System in November, 2006.
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