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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Updated: Jul 3, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

[Molecular characterization of vancomycin-resistant enterococci isolates].

Ying-mei Liu1, Bin Cao, Hui Wang

  • 1Department of Infectious Diseases and Clinical Microbiology, Beijing Chaoyang Hospital Affiliated to the Capital University of Medical Sciences, Beijing 100020, China.

Zhonghua Yi Xue Za Zhi
|August 8, 2008
PubMed
Summary
This summary is machine-generated.

Vancomycin-resistant enterococci (VRE) strains in Beijing Chaoyang Hospital primarily exhibit vanA and vanB genotypes. Enterococcus faecalis VRE is monoclonal, while Enterococcus faecium VRE is polyclonal, with some strains showing mixed resistance genotypes.

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Last Updated: Jul 3, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

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Published on: March 3, 2023

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Context:

  • Vancomycin-resistant enterococci (VRE) pose a significant threat in healthcare settings.
  • Understanding VRE prevalence and genetic characteristics is crucial for infection control.
  • This study focuses on VRE isolates from Beijing Chaoyang Hospital between 2003 and 2007.

Purpose:

  • To determine the prevalence and genotypes of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis.
  • To analyze the genotypic and phenotypic characteristics of VRE isolates.
  • To investigate the clonal relatedness of VRE strains.

Summary:

  • Thirty-eight VRE isolates were analyzed for prevalence and genotype. Most VRE strains were isolated from ICU patients.
  • Enterococcus faecalis isolates were predominantly vanB genotype, while Enterococcus faecium isolates were vanA genotype.
  • PFGE analysis revealed monoclonal VRE faecalis and polyclonal VRE faecium, with some Enterococcus faecium isolates showing incongruence between vancomycin resistance phenotype and genotype.

Impact:

  • Identifies the dominant VRE genotypes (vanA and vanB) circulating in a Beijing hospital.
  • Differentiates the population structure of VRE faecalis (monoclonal) and VRE faecium (polyclonal).
  • Highlights the occurrence of discordant glycopeptide resistance phenotypes and genotypes in VRE faecium, necessitating careful diagnostic interpretation.