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Related Experiment Video

Updated: Jun 23, 2026

Isolation of Murine Lymph Node Stromal Cells
05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

TSCOT+ thymic epithelial cell-mediated sensitive CD4 tolerance by direct presentation.

Sejin Ahn1, Gwanghee Lee, Soo Jung Yang

  • 1Laboratory of Cellular and Molecular Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, United States of America,

Plos Biology
|August 8, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed a mouse model to study thymic stromal cells and central tolerance. Introducing a foreign antigen into these cells efficiently established complete tolerance, suggesting therapeutic potential for deleting specific T cells.

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Last Updated: Jun 23, 2026

Isolation of Murine Lymph Node Stromal Cells
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11:17

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Published on: August 11, 2018

Area of Science:

  • Immunology
  • Cell Biology
  • Thymic Development

Background:

  • Mechanisms of central tolerance are not fully understood.
  • The specific role of thymic stromal cells in establishing tolerance is elusive.

Purpose of the Study:

  • To investigate the role of thymic stromal cells in central tolerance.
  • To characterize the function of thymic stromal cotransporter (TSCOT)-expressing cells.

Main Methods:

  • Developed a mouse model (TDLacZ) expressing LacZ antigen in TSCOT+ thymic stromal cells.
  • Analyzed T cell repertoire diversity and immune responses against LacZ.
  • Utilized fetal thymic reaggregate culture and TCR transgenic mice.

Main Results:

  • TDLacZ mice exhibited significant tolerance to LacZ antigen, indicated by a diverse T cell repertoire.
  • Tolerance was partially independent of Autoimmune Regulatory Element gene expression.
  • Crossing TDLacZ mice with anti-LacZ TCR transgenic mice led to complete deletion of double-positive thymocytes.
  • Experiments excluded cross-presentation by dendritic cells and medullary epithelial cells.

Conclusions:

  • Introduction of a neoantigen into TSCOT-expressing cells can induce robust central tolerance.
  • TSCOT+ cells play a critical role in establishing immune tolerance.
  • This approach may offer a strategy for deleting antigen-specific T cells, with potential therapeutic applications.