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Adsorption Isotherms II

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Brunauer, Emmett, and Teller (BET) introduced a theory in 1938 that modified Langmuir's assumptions to explain multilayer physical adsorption. This theory is applicable to Type II isotherms and provides a more realistic picture of adsorption processes. The BET theory assumes a uniform solid surface with localized adsorption sites, where adsorption at one site doesn't affect adsorption at neighboring sites. This theory also allows for the possibility of additional molecules being adsorbed on top...

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The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:

The Equilibrium Binding Constant and Binding Strength

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Adsorption Isotherms I

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Updated: Jul 3, 2026

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method

Published on: April 11, 2020

DNA binding during expanded bed adsorption and factors affecting adsorbent aggregation.

A Arpanaei¹, N Mathiasen, T J Hobley

¹Center for Microbial Biotechnology, Building 223, Institute for Systems Biology, Technical University of Denmark, 2800-Kgs. Lyngby, Denmark.

Journal of Chromatography. A

|August 8, 2008

Summary

Adding salt to DNA solutions can increase DNA binding capacity in anion exchange chromatography. However, this may reduce overall performance and protein capture in expanded bed adsorption chromatography.

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Monitoring Protein Adsorption with Solid-state Nanopores

Monitoring Protein Adsorption with Solid-state Nanopores

Published on: December 2, 2011

Area of Science:

Biotechnology
Chromatography
Bioprocessing

Background:

Expanded bed adsorption chromatography (EBA) is crucial for direct capture of biomolecules from complex feedstocks.
DNA can induce aggregation and bed contraction in chromatography, impacting process efficiency.
Understanding DNA-protein interactions is vital for optimizing bioseparation processes.

Purpose of the Study:

To investigate the impact of salt concentration and adsorbent ligand density on DNA-induced bed aggregation and contraction in EBA.
To determine the effect of these parameters on DNA binding capacity.
To evaluate the implications for protein capture in real feedstocks.

Main Methods:

Utilized strong anion exchanger Q HyperZ and calf thymus DNA in varying NaCl concentrations.
Employed two adsorbent batches with different ionic capacities (ligand densities).
Measured dynamic binding capacities and observed bed contraction during DNA loading.

Main Results:

Highest DNA binding capacities were achieved at 0.25-0.35M NaCl for low and high ligand density adsorbents, respectively.
Bed contraction occurred but did not correlate with binding capacity or DNA load concentration.
Bed contraction was dependent on salt concentration and adsorbent ligand density.

Conclusions:

Ligand density significantly influences the salt tolerance of anion exchangers for DNA binding.
Increasing salt to mitigate aggregation may paradoxically increase DNA binding, reducing EBA performance for protein capture.
Feedstock conditioning with salt needs careful consideration to avoid compromising protein recovery in EBA.