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Published on: February 17, 2023
Synthesis and characterization of BODIPY-labeled colchicine
Leggy A Arnold1, Patricia Ranaivo, R Kiplin Guy
1St. Jude Children's Research Hospital, Department of Chemical Biology and Therapeutics, 332 N. Lauderdale St., Mail Stop 1000, Memphis, TN 38105-2794, USA.
Abstract:
Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.
