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Related Experiment Videos

The Chlorella H+/hexose cotransporter gene.

K Wolf1, W Tanner, N Sauer

  • 1Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Federal Republic of Germany.

Current Genetics
|March 1, 1991
PubMed
Summary
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Researchers sequenced the Chlorella kessleri H+/hexose cotransporter (HUP1) gene, revealing a regular intron/exon pattern and biased codon usage. This study details the gene

Area of Science:

  • * Molecular Biology
  • * Genomics
  • * Biochemistry

Background:

  • * The Chlorella kessleri H+/hexose cotransporter (HUP1) facilitates sugar uptake in algae.
  • * Understanding the HUP1 gene's structure and regulation is crucial for algal biotechnology.
  • * Previous studies have not fully elucidated the complete genomic sequence of HUP1.

Purpose of the Study:

  • * To obtain the complete genomic sequence of the inducible Chlorella kessleri H+/hexose cotransporter (HUP1) gene.
  • * To analyze the intron-exon structure and codon usage bias of the HUP1 gene.
  • * To identify a consensus sequence for algal polyadenylation signals.

Main Methods:

  • * Isolation of overlapping clones from a lambda gt10 library.
  • * Determination of the complete genomic sequence of HUP1.

Related Experiment Videos

  • * Bioinformatic analysis of gene structure, intron lengths, and codon usage.
  • * Comparison of identified sequences with existing algal cDNA and polyadenylation data.

    • Main Results:

    • * The complete genomic sequence of the inducible Chlorella kessleri HUP1 gene was obtained.
    • * The HUP1 gene contains 14 introns, with a regular intron/exon pattern and an average intron length of 220 bp.
    • * Codon usage in the HUP1 gene is strongly biased, favoring C and suppressing A.
    • * A putative algal polyadenylation consensus sequence was identified and compared to other algal sequences.

    Conclusions:

    • * The detailed genomic sequence and structural analysis of the HUP1 gene provide fundamental insights into algal gene organization.
    • * The observed codon usage bias may reflect specific translational efficiency mechanisms in Chlorella kessleri.
    • * Identification of a consensus polyadenylation signal contributes to understanding gene expression regulation in algae.