Related Experiment Video
Updated: Jul 3, 2026
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
Published on: May 19, 2023
[The change of immunoactivity of dendritic cells induced by recombinant rat 4-1BBL]
Jian-Xin Tan1, Wei-Chang Chen, Xue-Guang Zhang
1Department of Gastroenterology in First Hospital, Suzhou 215006, China. yjx13906210479@sina.com
Aim:
To investigate the change of immunoactivity and surface marker of dendritic cells (DCs) derived from bone marrow and induced by rat 4-1BBL.
Methods:
Plasmid pIRES2-EGFP-4-1BBL was constructed and tansfected into HepG2 cells by lipofectin-mediated method, and positive cells were screened by G418. The expression of 4-1BBL in cells were detected by RT-PCR, and the expression of EGFP was detected by fluorescence microscopy. Various HepG2 cells were co-cultured with DC for 2 days, and then the expression of MHC-II and CD80 on DC were detected by FCM, and IL-6 and IL-12 levels secreted by DCs were tested by ELISA.
Results:
The recombinant pIRES2-EGFP-4-1BBL vector was successfully constructed and was steadily expressed in HepG2 cells transfected by pIRES2-EGFP-4-1BBL. Compared with HepG2 cells transfected by pIRES2-EGFP plasmid , recombinant HepG2 cells induced high expression of MHC-II and CD80 and secreted more IL-6 and IL-12 on DCs(P<0.05).
Conclusion:
The recombinant rat 4-1BBL could promote the maturity of DCs derived from bone marrow.
