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Updated: Jul 3, 2026
The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
[Construction and expression of non-fusional expression vector of the multi-epitope gene of hepatitis B virus]
Li-Min Luo1, Hu Xia, Zhuo-Liang Li
1Medical Center of Liver Diseases, 458 Hospital, Guangzhou 510602, China.
Aim:
To study the cloning, expression and antigen of therapeutic multi-epitope gene of hepatitis B virus.
Methods:
The multi-epitope gene of hepatitis B virus(BPT) was designed, synthesized and cloned into the prokaryotic expression vector pBAD/gIIIA. Then it was transformed into E.coli Top10 and multi-epitope protein of hepatitis B virus(B-BPT) was expressed under the induction of Arabinose. The immunogenicity of the protein was analyzed by Western blot detection.
Results:
The recombinant plasmid pBAD/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E.coli. Western blot detection showed the protein had ideal antigenicity.
Conclusion:
The design of therapeutic multi-epitope gene of hepatitis B virus is proved to be correct. The expressed protein may be a good therapeutic vaccine.
