A solid-in-oil nanodispersion for transcutaneous protein delivery.
Yoshiro Tahara1, Shota Honda, Noriho Kamiya
1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan.
Summary
Solid-in-oil nanodispersions effectively deliver hydrophilic proteins through the skin. This novel approach enhances transcutaneous protein delivery, maintaining protein function after permeation.
Area of Science:
- Biotechnology
- Materials Science
- Dermatology
Background:
- Transcutaneous drug delivery is challenging for hydrophilic macromolecules.
- Effective delivery of proteins via skin remains an unmet need.
Purpose of the Study:
- To evaluate solid-in-oil (S/O) nanodispersions for transcutaneous protein delivery.
- To demonstrate enhanced skin permeation of various model proteins.
Main Methods:
- Formulation of S/O nanodispersions containing model proteins (insulin, EGFP, HRP).
- In vitro permeation studies using Yucatan micropig skin.
- Assessment of protein integrity and function post-permeation.
Main Results:
- S/O nanodispersions facilitated the permeation of insulin, EGFP, and HRP through the stratum corneum.
- Delivered EGFP retained green fluorescence, and HRP retained catalytic activity.
- Proteins permeated the skin in a functional state.
Conclusions:
- S/O nanodispersions are a promising vehicle for transcutaneous delivery of hydrophilic proteins.
- This technology offers a potential solution for non-invasive protein-based therapies.
- Functional protein delivery via the skin is achievable with S/O nanodispersions.
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