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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

Updated: Jul 2, 2026

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers
06:34

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

Published on: December 1, 2017

Immunoassay for human serum hepcidin.

Tomas Ganz1, Gordana Olbina, Domenico Girelli

  • 1Intrinsic LifeSciences LLC, La Jolla, CA, USA. tganz@mednet.ucla.edu

Blood
|August 12, 2008
PubMed
Summary

A new assay accurately measures serum hepcidin, the key iron hormone. This breakthrough allows better understanding of iron disorders by reflecting physiological and pathological influences on hepcidin levels.

Area of Science:

  • Biochemistry
  • Endocrinology
  • Clinical Chemistry

Background:

  • Hepcidin is the primary hormone regulating iron metabolism.
  • Measuring hepcidin has been challenging, hindering research and clinical application.
  • Accurate hepcidin measurement is crucial for diagnosing and managing iron-related disorders.

Purpose of the Study:

  • To develop and validate the first serum enzyme-linked immunosorbent assay (ELISA) for hepcidin.
  • To establish reference ranges for serum hepcidin in healthy men and women.
  • To assess the correlation of serum hepcidin with urinary hepcidin and serum ferritin.

Main Methods:

  • Development and validation of a novel serum enzyme-linked immunosorbent assay (ELISA) for hepcidin.
  • Measurement of serum hepcidin concentrations in healthy male and female volunteers.

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  • Correlation analysis between serum hepcidin, urinary hepcidin, and serum ferritin levels.
  • Evaluation of hepcidin levels in various clinical conditions associated with iron disturbances.

    • Main Results:

    • The developed serum hepcidin ELISA demonstrated accurate and reproducible measurements with a lower limit of detection of 5 ng/mL.
    • Reference ranges for serum hepcidin were established: 29-254 ng/mL in men and 17-286 ng/mL in women.
    • Serum hepcidin correlated well with urinary hepcidin (r = 0.82) and serum ferritin (r = 0.63).
    • Distinct hepcidin alterations were observed in conditions like iron deficiency anemia, hemochromatosis, inflammation, multiple myeloma, and chronic kidney disease.

    Conclusions:

    • The novel serum hepcidin ELISA is a reliable tool for measuring hepcidin.
    • This assay provides valuable insights into iron homeostasis by reflecting physiological, pathological, and genetic factors.
    • The assay aids in diagnosing and understanding the etiology of various iron disorders.