PhosphoPOINT: a comprehensive human kinase interactome and phospho-protein database.

Area of Science:

• Biochemistry
• Molecular Biology
• Bioinformatics

Background:

• Protein kinases regulate biological processes by phosphorylating substrates, but many kinase substrates and motifs remain unidentified.
• Existing protein-protein interaction (PPI) datasets offer a potential avenue for discovering novel kinase-substrate relationships.
• Understanding kinase function is crucial for deciphering cellular signaling pathways.

Purpose of the Study:

• To develop a comprehensive database, PhosphoPOINT, for human kinase interactome and phospho-protein information.
• To facilitate the identification of potential kinase-substrate pairs using PPI data.
• To accelerate the functional characterization of kinase-mediated signaling.

Main Methods:

• Compiled a database of 4195 phospho-proteins and 15,738 phosphorylation sites.
• Annotated interactions among kinases, substrates, and interacting proteins.
• Integrated gene expression profiles, Gene Ontology cellular component data, and cSNPs for analysis.

Main Results:

• PhosphoPOINT provides robust annotation for kinases, substrates, and interacting proteins.
• Identified 64 phosphorylation sites linked to disease phenotypes, including schizophrenia and hypertension.
• Incorporated a search function for phospho-peptides based on known kinase/phosphatase motifs.

Conclusions:

• PhosphoPOINT enhances the discovery of kinase-substrate interactions by leveraging PPI data.
• The database aids in understanding kinase function and its role in human diseases.
• PhosphoPOINT is a valuable resource for researchers studying kinase signaling pathways.