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Updated: Jul 2, 2026

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

Ceramide kinase and the ceramide-1-phosphate/cPLA2alpha interaction as a therapeutic target

Nadia F Lamour1, Charles E Chalfant

  • 1Department of Biochemistry and Molecular Biology, Virginia Commonwealth University-School of Medicine, Richmond, VA 23298-0614, USA.

Current Drug Targets
|August 12, 2008
PubMed

Insights

Ceramide kinase (CERK) and its product ceramide-1-phosphate (C1P) are involved in inflammation, immunity, and cancer. This review overviews C1P biosynthesis and its role in activating cPLA2alpha for eicosanoid synthesis.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Ceramide kinase (CERK) has been studied for over a decade.
  • CERK plays roles in inflammation, immunity, and cancer signaling pathways.
  • Ceramide-1-phosphate (C1P) is a key product of CERK activity.

Purpose of the Study:

  • To review the biosynthesis of ceramide-1-phosphate (C1P).
  • To overview the biological roles of CERK and C1P.
  • To focus on C1P's role in eicosanoid synthesis via cPLA2alpha activation.

Main Methods:

  • Literature review of published reports on CERK and C1P.
  • Analysis of signaling pathways involving CERK and C1P.
  • Discussion of therapeutic implications.

Main Results:

  • CERK produces C1P, a lipid mediator.
  • C1P is involved in activating cytosolic phospholipase A2-alpha (cPLA2alpha).
  • Activation of cPLA2alpha by C1P influences eicosanoid synthesis.

Conclusions:

  • CERK and C1P are significant in cellular signaling.
  • C1P's role in cPLA2alpha activation is crucial for eicosanoid production.
  • Inhibitors targeting these pathways may have therapeutic potential.

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