Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 2, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Dividable membrane with multi-reaction wells for microarray biochips.

Yaw-Jen Chang1, Chih-Yu Hu, Li-Te Yin

  • 1Department of Mechanical Engineering, Chung Yuan Christian University, 200 Chung Pei Rd., Chung Li, Taiwan 32023, ROC. justin@cycu.edu.tw

Journal of Bioscience and Bioengineering
|August 12, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Unique <i>SLC26A4</i> Mutation Spectrum in a Mongolian Enlarged Vestibular Aqueduct Cohort via Whole-Exome Sequencing: A Preliminary Study.

International journal of molecular sciences·2026
Same author

Endoscopic Type I Tympanoplasty: Comparative Outcomes of Autograft, Allograft, and Xenograft.

Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale·2026
Same author

Construction and validation of machine learning models combining clinical data and radiological characteristics for early identification of reoperations for deep neck infection.

American journal of otolaryngology·2026
Same author

Association of GJB2 P.V37I With Sudden Sensorineural Hearing Loss and Endoplasmic Reticulum Stress.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2026
Same author

Machine Learning Prediction of Progression to Dialysis in Patients With Polycystic Kidney Disease: Population-Based Retrospective Cohort Study.

JMIR medical informatics·2026
Same author

From Young-to older-elderly: shifting casualty patterns in traffic accidents within a motorcycle-oriented transport environment.

International journal of injury control and safety promotion·2026
Same journal

Three carbohydrate-binding modules of laminarinase LA-Lam recognize β-1,3-glucan and enhance hydrolytic activity.

Journal of bioscience and bioengineering·2026
Same journal

Cre-loaded integrase-defective lentiviral vectors for targeted cassette exchange in CHO cells.

Journal of bioscience and bioengineering·2026
Same journal

Structure- and phylogeny-guided redirection of substrate specificity in diaminobutyric acid racemase PddB toward ornithine and lysine.

Journal of bioscience and bioengineering·2026
Same journal

Identification of key enzymes involved in 1,2,4-trihydroxybenzene metabolism in the hyper lignin-degrading fungus Phanerochaete sordida YK-624.

Journal of bioscience and bioengineering·2026
Same journal

Adaptive laboratory evolution of Methylorubrum extorquens for lanthanide-dependent robust growth under high concentrations of methanol.

Journal of bioscience and bioengineering·2026
Same journal

Cas9-PALB2 fusion protein enhances CRISPR/Cas9 mediated gene knock-in efficiency.

Journal of bioscience and bioengineering·2026
See all related articles

This study introduces a novel polydimethylsiloxane (PDMS) multi-well membrane for microarray biochips, enabling dividable incubation chambers. The optimized fabrication ensures excellent physical absorption, preventing cross-contamination for sensitive alpha-1-fetoprotein (AFP) detection.

Area of Science:

  • Biotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Microarray biochips require precise control over sample containment to prevent cross-contamination.
  • Polydimethylsiloxane (PDMS) is a versatile material for microfluidic device fabrication.
  • Dividable incubation chambers enhance sample throughput and reduce reagent consumption.

Purpose of the Study:

  • To develop and optimize a polydimethylsiloxane (PDMS) multi-well membrane for microarray biochips.
  • To investigate fabrication parameters for optimal physical absorption of the PDMS membrane onto a chip substrate.
  • To validate the performance of the multi-well chip using immunoassays for alpha-1-fetoprotein (AFP) detection.

Main Methods:

  • Fabrication of a multi-well membrane using polydimethylsiloxane (PDMS).

More Related Videos

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
07:56

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Related Experiment Videos

Last Updated: Jul 2, 2026

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method
07:56

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

  • Optimization of PDMS forming temperature, time, and material mixing proportions.
  • Immunoassays, specifically an alpha-1-fetoprotein (AFP) antigen sandwich experiment, were conducted to verify chip performance.

    • Main Results:

    • The fabricated multi-well membrane demonstrated excellent physical absorption onto the chip substrate.
    • Immunoassays for AFP achieved a detection limit of 10 ng/ml.
    • The dynamic range for AFP detection was determined to be 30-3000 ng/ml.

    Conclusions:

    • The optimized PDMS multi-well membrane effectively provides dividable incubation chambers on a single biochip.
    • The biochip design minimizes cross-contamination and interference between samples.
    • This technology facilitates efficient surface utilization and supports multiple-sample experiments, enhancing diagnostic capabilities.