Related Experiment Video
Updated: Jul 2, 2026
08:23
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Endothelial nitric oxide (NO) and its pathophysiologic regulation.
Anuran Chatterjee1, Stephen M Black, John D Catravas
1Pulmonary Vascular Disease Program, Vascular Biology Center, Medical College of Georgia, Augusta, Georgia 30912-2500, USA.
Vascular Pharmacology
|August 12, 2008
Summary
Nitric oxide (NO) is vital for vascular health. Heat shock protein 90 (hsp90) regulates nitric oxide synthases (NOS), impacting cardiovascular diseases like atherosclerosis and hypertension.
Area of Science:
- Cardiovascular Science
- Molecular Biology
- Biochemistry
Background:
- Nitric oxide (NO) is a critical signaling molecule in vascular function.
- Three isoforms of nitric oxide synthases (NOS) generate NO: nNOS, iNOS, and eNOS.
- Endothelial NOS (eNOS) dysfunction is implicated in cardiovascular diseases.
Purpose of the Study:
- To review the transcriptional regulation of eNOS.
- To examine factors affecting eNOS activity and function.
- To focus on the role of heat shock protein 90 (hsp90) in NO-associated vascular pathologies.
Main Methods:
- Literature review of transcriptional regulation of eNOS.
- Analysis of factors influencing eNOS activity.
- Examination of hsp90's role in NOS function and vascular disease.
Main Results:
- eNOS expression and activity are altered in cardiovascular diseases.
- hsp90 associates with NOS, influencing its folding and function.
- Modulating hsp90-NOS interaction shows therapeutic potential for vascular diseases.
Conclusions:
- Altered NOS function contributes to vascular pathologies.
- hsp90 plays a regulatory role in NO-associated pathogenesis.
- Targeting the hsp90-NOS pathway offers therapeutic strategies for cardiovascular diseases.
More Related Videos
Related Concept Videos
01:28Nitric Oxide Signaling Pathway
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
01:24Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
01:26Coronary Artery Disease II: Pathophysiology
Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
01:23Antihypertensive Drugs: Vasodilators
Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...
01:21Paracrine Signaling
Paracrine signaling allows cells to communicate with their immediate neighbors via secretion of signaling molecules. Such a signal can only trigger a response in nearby target cells because the signal molecules degrade quickly or are inactivated if not taken up. Prominent examples of paracrine signaling include nitric oxide signaling in blood vessels, synaptic signaling of neurons, the blood clotting system, tissue repair/wound healing, and local allergic skin reactions. Nitric oxide as a...
01:29Hypertension II: Pathophysiology
Hypertension is a chronic condition in which the blood's force against artery walls is excessively high, posing risks such as heart disease. The condition's underlying mechanisms involve complex interactions among the cardiovascular, kidney, and autonomic nervous systems.Renin-Angiotensin-Aldosterone System (RAAS): This system significantly influences blood pressure regulation. When blood pressure decreases, the kidneys secrete renin. This enzyme transforms angiotensinogen, a plasma protein,...