Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Garment-related patient-reported outcomes: a longitudinal pre-post comparison of autologous versus implant-based breast reconstruction.

European journal of oncology nursing : the official journal of European Oncology Nursing Society·2026
Same author

Orexin-A Inhibits Lipopolysaccharide-Induced Cell Migration in Cultured Mouse Astrocytes via Activation of Orexin 1 Receptor: Involvement of GABA Signaling.

Journal of cellular biochemistry·2026
Same author

Corrigendum to "Sonocatalytic degradation of bisphenol A in aqueous solution: A review". [Ultrason. Sonochemistry 125 (2026) 107745].

Ultrasonics sonochemistry·2026
Same author

Sonocatalytic degradation of bisphenol A in aqueous solution: A review.

Ultrasonics sonochemistry·2026
Same author

Coarse-Grained Molecular Dynamics Simulations of Lipid Nanodroplets and Endosomal Membranes: Focusing on the Fusion Mechanisms.

International journal of molecular sciences·2025
Same author

Harnessing theta-gamma coupled brainwaves using ultrasound for spinal astrocyte revitalization and sustained neuropathic pain relief in mice.

Nature communications·2025

Related Experiment Video

Updated: Jul 2, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

Effect of normalization methods on biomarker identification utilizing mass spectrometry.

Hyunjin Shin1, Chih-Wen Kan, Harrison J Hocker

  • 1Department of Biostatistics and Computational Biology Dana-Farber Cancer Institute, Boston, MA, USA.

AMIA ... Annual Symposium Proceedings. AMIA Symposium
|August 13, 2008
PubMed
Summary

Different normalization methods significantly impact biomarker discovery in mass spectrometry. This study reveals that various techniques lead to the selection of distinct features with varying discriminatory abilities, affecting results.

More Related Videos

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
08:27

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Published on: July 27, 2021

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Related Experiment Videos

Last Updated: Jul 2, 2026

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
08:27

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization

Published on: July 27, 2021

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Area of Science:

  • Biomarker discovery
  • Analytical chemistry
  • Mass spectrometry data analysis

Background:

  • Mass spectrometry is crucial for biomarker identification.
  • Normalization is essential for comparing mass spectra across samples.
  • The comparative impact of different normalization methods remains largely uninvestigated.

Purpose of the Study:

  • To systematically compare widely used normalization methods in mass spectrometry.
  • To investigate the influence of normalization techniques on biomarker identification.
  • To understand how different normalization strategies affect feature selection and discriminatory power.

Main Methods:

  • Comparative analysis of prevalent normalization techniques.
  • Systematic evaluation of normalization impact on mass spectrometry data.
  • Assessment of feature selection and discriminatory power across methods.

Main Results:

  • Normalization methods demonstrably influence the selection of biomarkers.
  • Different normalization techniques result in the identification of distinct sets of features.
  • The discriminatory power of selected features varies significantly based on the normalization method used.

Conclusions:

  • The choice of normalization method is a critical factor in mass spectrometry-based biomarker discovery.
  • Understanding the impact of normalization is essential for reproducible and reliable results.
  • Further research should guide the selection of optimal normalization strategies for specific applications.