Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A facile method for simultaneously measuring neuronal cell viability and neurite outgrowth.

Current chemical genomics and translational medicine·2015

Same author

BacMam-mediated gene delivery into multipotent mesenchymal stromal cells.

Methods in molecular biology (Clifton, N.J.)·2011

Same author

Development of a GPR23 cell-based β-lactamase reporter assay.

Methods in enzymology·2010

Same author

Differential effects of allosteric M(1) muscarinic acetylcholine receptor agonists on receptor activation, arrestin 3 recruitment, and receptor downregulation.

ACS chemical neuroscience·2010

Same author

Cell-based potassium ion channel screening using the FluxOR assay.

Journal of biomolecular screening·2010

Same author

Utilization of the Tango beta-arrestin recruitment technology for cell-based EDG receptor assay development and interrogation.

Journal of biomolecular screening·2009

Search research articles

Related Experiment Video

Updated: Jul 2, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Fluorescent probes for cellular assays.

George T Hanson¹, Bonnie J Hanson

¹Invitrogen Discovery Sciences, Madison, WI 53719, USA.

Combinatorial Chemistry & High Throughput Screening

|August 13, 2008

Summary

This summary is machine-generated.

This review covers fluorescent probes and assays, highlighting their increasing use in cellular studies for pharmaceutical research. These sensitive tools offer a safer alternative to radioactive methods for biological specimen analysis.

More Related Videos

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Related Experiment Videos

Last Updated: Jul 2, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

Area of Science:

Biochemistry
Cell Biology
Analytical Chemistry

Background:

Fluorescent probes, fluorophores localizing to specific biological regions or responding to stimuli, have been vital in cellular process studies for a century.
Growing safety and environmental concerns regarding radioactive probes have increased the popularity of fluorescent probes.
Cellular assays are increasingly utilized in biological and pharmaceutical research.

Purpose of the Study:

To provide a comprehensive overview of fluorescent probe types.
To review various fluorescent assay methodologies.
To discuss the application of fluorescent probes and assays in cellular studies for pharmaceutically relevant targets.

Main Methods:

Literature review of fluorescent probe and assay technologies.
Analysis of applications in pharmaceutical target identification and validation.
Synthesis of information on probe localization and stimulus response.

Main Results:

Fluorescent probes offer high sensitivity and selectivity for studying cellular processes.
They serve as a safer alternative to radioactive probes in biological research.
Diverse fluorescent probes and assays are applicable to various pharmaceutical targets.

Conclusions:

Fluorescent probes and assays are essential tools in modern cellular research.
Their application spans a wide range of pharmaceutical targets, enhancing drug discovery.
The trend towards fluorescent methods reflects a need for safer, sensitive biological detection.