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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Label-free cell-based functional assays.

Lisa K Minor1

  • 1Johnson & Johnson Pharmaceutical Research Institute, Department of Drug Discovery, Spring House, PA 19477, USA. lminor@prdus.jnj.com

Combinatorial Chemistry & High Throughput Screening
|August 13, 2008
PubMed
Summary

Label-free assays using electrical impedance or refractive index offer novel, dye-free methods for cell-based screening. These techniques enable kinetic measurements of cellular functions like proliferation and apoptosis, advancing drug discovery.

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Area of Science:

  • Biotechnology and Pharmaceutical Sciences
  • Cellular and Molecular Biology
  • Assay Development

Background:

  • Emergence of label-free technologies for cell-based assays.
  • These methods utilize electrical impedance or refractive index to measure cellular responses.
  • Growing interest due to the ability to detect phenotypic changes without dyes or cell lysis.

Purpose of the Study:

  • To describe novel label-free technologies for cell-based functional response measurement.
  • To highlight applications in high-throughput screening (HTS), directed screening, and compound profiling.
  • To discuss the utility and limitations of these assays for various receptor target classes.

Main Methods:

  • Utilizing electrical impedance and refractive index as label-free detection modalities.
  • Measuring kinetic cellular responses, including proliferation, attachment, spreading, and apoptosis.
  • Application to cell-based assays involving native protein expression.

Main Results:

  • Demonstrated label-free assays can effectively measure key cellular functions.
  • These methods allow for kinetic analysis, providing rich assay content.
  • Successful application across different receptor target classes, such as receptor tyrosine kinases and G protein-coupled receptors.

Conclusions:

  • Label-free technologies represent a significant advancement in cell-based functional assays.
  • They offer a versatile, dye-free approach for screening and profiling compounds.
  • These methods hold considerable potential for drug discovery and development pipelines.