Related Concept Videos

Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa03:04

Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa

Source: Rugjee, K. N. et. al., Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa. J. Vis. Exp. (2016)This video demonstrates the generation of a cyclic-di-GMP-responsive reporter strain in Pseudomonas aeruginosa using electroporation. Successful transformation is confirmed through antibiotic selection and fluorescence-based...
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Swarming Behavior of Pseudomonas aeruginosa Under Antibiotic Stress02:38

Swarming Behavior of Pseudomonas aeruginosa Under Antibiotic Stress

Source: Bru, J., et al., Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. J. Vis. Exp. (2020)This video demonstrates the procedure for analyzing the swarming behavior of Pseudomonas aeruginosa by observing bacterial growth from a central culture toward satellite spots containing the same culture mixed with an antibiotic, using an agar plate setup and time-lapse...
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Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa05:09

Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa

This protocol provides both qualitative and quantitative analyses of total siderophores, pyoverdine, and pyochelin from Pseudomonas...
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Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Pseudomonas aeruginosa infection causes significant morbidity in vulnerable hosts. The nonredundant transposon insertion mutant library of P. aeruginosa strain PA14, designated as PA14NR Set, facilitates analysis of gene functionality in numerous processes. Presented here is a protocol to generate high-quality copies of the PA14NR Set mutant...
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A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos02:46

A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos

The video demonstrates the phage therapy technique targeting Pseudomonas aeruginosa infection in cystic fibrosis zebrafish embryos. Phage therapy effectively diminishes bacterial load and mitigates proinflammatory immune responses in CF zebrafish embryos infected with bacteria, leading to reduced CF embryo...
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An In Vitro Technique to Study Antimicrobial Treatments on Bacterial Aggregates04:12

An In Vitro Technique to Study Antimicrobial Treatments on Bacterial Aggregates

This video demonstrates an in vitro technique to study the impact of antimicrobial treatment on Pseudomonas aeruginosa (Pa) aggregates. Fluorescently-labeled Pa cells are grown as aggregates, mimicking the growth pattern during infection. Upon challenging the aggregates with a sublethal concentration of the antibiotic colistin, the real-time assessment of the bacteria-antibiotic interaction is performed via fluorescence...
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