Related Experiment Video
Updated: Jan 20, 2026

05:09
Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control
Published on: March 15, 2024
5.9K
Antimicrobial studies with the Pseudomonas aeruginosa two-allele library require caution
Antimicrobial Agents and Chemotherapy
|August 13, 2008
Abstract
No abstract available in PubMed .
Related Concept Videos
Generation of a Fluorescent Reporter Strain in Pseudomonas aeruginosa
399
Source: Rugjee, K. N. et. al., Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa. J. Vis. Exp. (2016)This video demonstrates the generation of a cyclic-di-GMP-responsive reporter strain in Pseudomonas aeruginosa using electroporation. Successful transformation is confirmed through antibiotic selection and fluorescence-based...
399
Swarming Behavior of Pseudomonas aeruginosa Under Antibiotic Stress
267
Source: Bru, J., et al., Time-lapse Imaging of Bacterial Swarms and the Collective Stress Response. J. Vis. Exp. (2020)This video demonstrates the procedure for analyzing the swarming behavior of Pseudomonas aeruginosa by observing bacterial growth from a central culture toward satellite spots containing the same culture mixed with an antibiotic, using an agar plate setup and time-lapse...
267
05:09Qualitative and Quantitative Analysis of Siderophore Production from Pseudomonas aeruginosa
5.9K
This protocol provides both qualitative and quantitative analyses of total siderophores, pyoverdine, and pyochelin from Pseudomonas...
5.9K
11:35Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
8.6K
Pseudomonas aeruginosa infection causes significant morbidity in vulnerable hosts. The nonredundant transposon insertion mutant library of P. aeruginosa strain PA14, designated as PA14NR Set, facilitates analysis of gene functionality in numerous processes. Presented here is a protocol to generate high-quality copies of the PA14NR Set mutant...
8.6K
02:46A Phage Therapy against Pseudomonas aeruginosa Infection in Zebrafish Embryos
502
The video demonstrates the phage therapy technique targeting Pseudomonas aeruginosa infection in cystic fibrosis zebrafish embryos. Phage therapy effectively diminishes bacterial load and mitigates proinflammatory immune responses in CF zebrafish embryos infected with bacteria, leading to reduced CF embryo...
502
04:12An In Vitro Technique to Study Antimicrobial Treatments on Bacterial Aggregates
623
This video demonstrates an in vitro technique to study the impact of antimicrobial treatment on Pseudomonas aeruginosa (Pa) aggregates. Fluorescently-labeled Pa cells are grown as aggregates, mimicking the growth pattern during infection. Upon challenging the aggregates with a sublethal concentration of the antibiotic colistin, the real-time assessment of the bacteria-antibiotic interaction is performed via fluorescence...
623

