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Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)
Beatriz Águeda1, Javier Parladé2, Luz Marina Fernández-Toirán3,4
1Departamento de Investigación y Experiencias Forestales de Valonsadero, Consejería de Medio Ambiente, Junta de Castilla y León. Apdo. de correos 175, 42080, Soria, Spain. aguherbe@jcyl.es.
Abstract:
Ectomycorrhizas of Boletus aereus, Boletus edulis, and Boletus reticulatus were synthesized with Cistus sp. under laboratory conditions using synthesis tubes filled with a mixture of sterilized peat-vermiculite and nutrient solution. The fungal strains isolated from sporocarps were identified by molecular techniques. The inoculated seedlings were grown for 4-5 months. The ectomycorrhizas formed were described based on standard morphological and anatomical characters. The three ectomycorrhizas described were very similar, with white monopodial-pinnate morphology, a three-layered plectenchymatous mantle on plan view and boletoid rhizomorphs.
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