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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

[Comparison of norovirus genotypes by real time PCR]

Masayuki Hara1, Etsuko Utagawa

  • 1Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases.

Kansenshogaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
|August 14, 2008
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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