Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Detailed Structure and Function of Lymph Nodes01:23

Detailed Structure and Function of Lymph Nodes

Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TRBC1 Immunohistochemistry Utilization in Formalin-fixed Paraffin-embedded Tissue as an Adjunct to T-cell Clonality Testing in the Assessment of Cutaneous Lymphoproliferative Disorders.

The American journal of surgical pathology·2026
Same author

Expression and prognostic value of cyclase-associated proteins in cutaneous melanoma.

Melanoma research·2026
Same author

Metaplastic Carcinoma of the Breast With Melanocytic Differentiation: Case Report With Molecular Analysis.

International journal of surgical pathology·2026
Same author

A complex case of chronic cutaneous <i>Borrelia burgdorferi</i> sensu stricto infection manifesting as morphea-like sclerosis in the United States.

JAAD case reports·2026
Same author

Multiple eruptive dermatofibromas occurring in association with monoclonal gammopathy of undetermined significance: A case report.

SAGE open medical case reports·2026
Same author

ICG-based fluorescence imaging to assess muscle perfusion for guiding surgical debridement after acute compartment syndrome.

Injury·2026

Related Experiment Video

Updated: Jul 2, 2026

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy
07:51

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy

Published on: June 1, 2019

False-positive cells in sentinel lymph nodes.

Jeoffry B Brennick1, Shaofeng Yan

  • 1Department of Pathology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire 03755, USA. Jeoffry.B.Brennick@Hitchcock.org

Seminars in Diagnostic Pathology
|August 14, 2008
PubMed
Summary

Sentinel lymph nodes (SLN) in melanoma patients require careful evaluation. Immunohistochemical stains can yield false positives, necessitating correlation with H+E staining to accurately detect metastatic melanoma.

More Related Videos

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

Related Experiment Videos

Last Updated: Jul 2, 2026

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy
07:51

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy

Published on: June 1, 2019

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

Area of Science:

  • Oncology
  • Pathology
  • Immunohistochemistry

Background:

  • Sentinel lymph node (SLN) biopsy is crucial for melanoma staging.
  • Standard SLN examination involves H+E staining and immunohistochemistry (IHC) for melanocytic markers.
  • Potential for false-positive results in IHC staining of SLN exists.

Purpose of the Study:

  • To highlight the challenges in interpreting IHC results for melanoma in SLN.
  • To discuss the origins of 'false-positive' cells in SLN.
  • To emphasize the importance of correlating H+E and IHC findings.

Main Methods:

  • Review of histopathological examination of melanoma SLN.
  • Analysis of immunohistochemical staining patterns using melanocytic markers (e.g., MART-1).
  • Correlation of H+E stained sections with IHC findings.

Main Results:

  • IHC staining can identify MART-1 positive cells in SLN unrelated to metastatic melanoma.
  • 'False-positive' cells may represent nodal nevi, cross-reacting antigens, phagocytosed antigens, or melanocytes.
  • Accurate diagnosis requires careful integration of H+E and IHC data.

Conclusions:

  • Interpretation of melanoma SLN requires meticulous correlation of H+E and IHC.
  • Understanding potential sources of false positives is key to accurate melanoma staging.
  • Histopathological expertise is vital for definitive SLN assessment in melanoma.