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Related Experiment Video

Updated: Jul 2, 2026

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
08:10

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Published on: November 28, 2018

Tracing functional antigen-specific CCR6 Th17 cells after vaccination.

Johann Pötzl1, Catherine Botteron, Eugen Tausch

  • 1Institute of Immunology, University of Regensburg, Regensburg, Germany.

Plos One
|August 14, 2008
PubMed
Summary

CC-chemokine receptor 6 (CCR6) marks functional T helper 17 (Th17) cells during immune responses. These cells are crucial early responders, and targeting them may prevent autoimmunity and enhance immunity.

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Area of Science:

  • Immunology
  • Cellular Biology
  • T cell differentiation

Background:

  • T helper cell function is location-dependent, with chemokine receptor expression guiding migration.
  • CC-chemokine receptor 6 (CCR6) is a marker for tissue-homing effector T cells, including T helper 17 (Th17) cells.
  • Th17 cells are implicated in autoimmunity and host defense, but their in vivo development remains unclear.

Purpose of the Study:

  • To investigate the development and function of Th17 cells during physiological immune responses in vivo.
  • To identify specific markers for functional Th17 cells in an active immune setting.
  • To explore the role of Th17 cells in autoimmune disease models.

Main Methods:

  • Analysis of Th17 cell development and function in immune-competent mice during an ongoing immune response.

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In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
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Published on: October 25, 2024

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Last Updated: Jul 2, 2026

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
08:10

Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector

Published on: November 28, 2018

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
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In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

  • Assessment of CCR6 expression and IL-17 production in T helper cells.
  • In vitro re-stimulation assays to evaluate antigen-specific Th17 cell responses.
  • Experimental autoimmune encephalomyelitis (EAE) model to assess the role of CCR6(+) Th17 cells in disease pathogenesis.

    • Main Results:

    • CCR6(+) T helper cells were found to produce IL-17 in both naive and vaccinated mice.
    • Activated CCR6(+) Th17 cells exhibited robust proliferation and expressed activation markers.
    • Vaccination induced antigen-specific CCR6(+) Th17 cells capable of cytokine production and proliferation upon re-stimulation.
    • Depletion of CCR6(+) Th cells ameliorated disease severity in a mouse model of multiple sclerosis (EAE).

    Conclusions:

    • CCR6 is identified as a specific marker for functional, antigen-specific Th17 cells during immune responses.
    • Th17 cell activation precedes Th1 cell differentiation, suggesting they act as early effector cells.
    • Targeting CCR6(+) Th17 cell activation presents a potential strategy for preventing autoimmunity and establishing protective immunity.