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Cell adhesion and proliferation are reduced on stainless steel coated with polysaccharide-based polymeric
Pasquale P Vicario1, Zichun Lu, Irina Grigorian
1Hydromer Inc, Branchburg, New Jersey 08876, USA. pvicario@hydromer.com
Abstract:
Hydromer's polymeric formulations F200 and F202 were evaluated after application to a synthetic substrate for effects on cell adhesion and proliferation. A significant reduction in cell adhesion was observed when cells grown on medical-grade stainless steel coated with these polymers were stained and examined under a fluorescence microscope. This reduction in cell adhesion/proliferation was confirmed when cells were isolated and analyzed by the MTS cell proliferation assay. The rate of growth of cells on F200- and F202-coated stainless steel monitored over a period of 7 days was significantly less than that observed on uncoated stainless steel, suggesting that the rate of growth of cells was reduced. The adhesion/proliferation of human umbilical vein endothelial cells (HUVEC) to coated substrates was also decreased significantly, indicating that the reduction in cell adhesion/proliferation is not restricted to only fibroblasts. Additional studies have indicated that the adhesion/proliferation of murine fibroblasts and human endothelial cells to stainless coated with a modified formulation exhibiting a high degree of lubricity was also significantly reduced. This lubricious formulation was also observed to be effective in reducing platelet adhesion, data supporting the view that lubricity also contributes to a reduction in cell and platelet adhesion. The application of these polymeric coatings on devices designed for medical implantation may not only prevent thrombus formation but may also retard the process of restenosis.
Insights
Polymeric coatings on medical-grade stainless steel significantly reduce cell adhesion and proliferation. This lubricity may prevent thrombus formation and restenosis in medical implants.
Area of Science:
- Biomaterials Science
- Medical Device Coatings
- Cell Biology
Background:
- Medical implants require biocompatible surfaces to prevent adverse cellular responses.
- Cell adhesion and proliferation on implant surfaces can lead to complications like restenosis and thrombus formation.
Purpose of the Study:
- To evaluate the impact of Hydromer's polymeric formulations (F200, F202) on cell adhesion and proliferation on stainless steel.
- To assess the effect of a lubricious polymer formulation on cell and platelet adhesion.
- To determine the potential of these coatings for medical implant applications.
Main Methods:
- Application of polymeric coatings (F200, F202) to medical-grade stainless steel substrates.
- Fluorescence microscopy to assess cell adhesion.
- MTS cell proliferation assay to quantify cell growth.
- Evaluation of human umbilical vein endothelial cells (HUVEC) and murine fibroblasts adhesion.
- Assessment of platelet adhesion on lubricious coated surfaces.
Main Results:
- Significant reduction in fibroblast and HUVEC adhesion and proliferation on polymer-coated stainless steel.
- Polymeric coatings significantly decreased cell growth rate over a 7-day period compared to uncoated controls.
- A lubricious polymer formulation markedly reduced both cell and platelet adhesion.
- Lubricity of the coating correlated with reduced cell and platelet adhesion.
Conclusions:
- Hydromer's polymeric coatings effectively inhibit cell adhesion and proliferation on stainless steel.
- The lubricious formulation demonstrates potential for reducing thrombus formation and restenosis.
- These findings suggest promising applications for these coatings in medical implant devices.
