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Updated: Jul 2, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Inkjet-printed microfluidic multianalyte chemical sensing paper.

Koji Abe1, Koji Suzuki, Daniel Citterio

  • 1Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.

Analytical Chemistry
|August 14, 2008
PubMed
Summary

This study introduces an all-inkjet-printed microfluidic paper device for simultaneous pH, protein, and glucose detection. This method enables quantitative analysis using a single printing apparatus and digital color analysis.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Microfluidic devices offer miniaturized platforms for chemical analysis.
  • Paper-based devices are low-cost and disposable alternatives for point-of-care diagnostics.
  • Simultaneous multianalyte detection is crucial for comprehensive diagnostic assessments.

Purpose of the Study:

  • To develop a novel inkjet printing method for fabricating integrated microfluidic paper-based analytical devices (µPADs).
  • To enable simultaneous, quantitative detection of pH, total protein, and glucose using colorimetric assays.
  • To demonstrate a single-apparatus fabrication process for paper-based chemical sensors.

Main Methods:

  • Fabrication of 3D hydrophilic microfluidic patterns and sensing areas on filter paper via inkjet etching of a hydrophobic poly(styrene) layer.

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Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Last Updated: Jul 2, 2026

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  • Printing of "chemical sensing inks" containing reagents for colorimetric assays using the same inkjet device.
  • Optimization of pattern and ink arrangement for homogeneous color responses.
  • Quantitative analysis of color changes in the L*a*b* color space using a color scanner and computer program.

    • Main Results:

    • Successfully fabricated "all-inkjet-printed" microfluidic paper devices capable of multianalyte sensing.
    • Demonstrated simultaneous determination of pH (5-9), total protein (0.46-46 µM human serum albumin), and glucose (2.8-28.0 mM).
    • Achieved quantitative results through digital color analysis, suitable for clinical urine analysis.

    Conclusions:

    • The developed inkjet printing technique provides a versatile and cost-effective method for producing integrated paper-based microfluidic sensing devices.
    • This approach facilitates the simultaneous quantitative measurement of multiple analytes, enhancing diagnostic capabilities.
    • The all-inkjet-printed sensors show potential for widespread application in point-of-care diagnostics and biochemical analysis.