Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Global subsidence of river deltas.

Nature·2026
Same author

International multicenter retrospective study on pleomorphic rhabdomyosarcoma (P-RMS), a PUSH platform study: outcome of primary localized disease.

ESMO open·2026
Same author

Global subsidence of river deltas.

Nature·2026
Same author

Working with Parry sound area local Canadian First Nations to describe a good death and ensure cultural sensitivity at the end-of-life.

BMC palliative care·2025
Same author

Adaptation and validation of perceived HIV and TB stigma scales among persons with TB.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2025
Same author

Trauma-informed HIV prevention for forcibly displaced adolescents and young adults.

The lancet. HIV·2025
Same journal

Compressed multi-scale entropy and its application in mechanical fault diagnosis.

The Review of scientific instruments·2026
Same journal

Bidirectional drive and multi-resolution adjustment across frequency bands in inertial impact piezoelectric motors via multimodal resonant vibration.

The Review of scientific instruments·2026
Same journal

A magnetic field sensor based on flaky Terfenol-D material and dual fiber grating.

The Review of scientific instruments·2026
Same journal

A novel E-field eight-way cavity combiner for high-power S-band applications.

The Review of scientific instruments·2026
Same journal

Constant radius blade spring suspended bench for vibration isolation.

The Review of scientific instruments·2026
Same journal

Qualification of infrared optical fibers and emitters for a spectrometer for in situ planetary exploration: Results from the TRIS (TRansmission and Illumination System) project.

The Review of scientific instruments·2026
See all related articles

Related Experiment Video

Updated: Jul 2, 2026

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

ENDOR cavity for electron spin echo experiments.

J L Davis1, W B Mims

  • 1Bell Laboratories, Murray Hill, New Jersey 07974.

The Review of Scientific Instruments
|August 1, 1978
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microwave cavity designed for Electron Nuclear Double Resonance (ENDOR) measurements, enhancing electron spin echo experiments. The cavity offers tunable parameters and efficient radio-frequency magnetic field generation for improved sensitivity.

More Related Videos

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Related Experiment Videos

Last Updated: Jul 2, 2026

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

Area of Science:

  • Spectroscopy
  • Physical Chemistry
  • Materials Science

Background:

  • Electron spin echo (ESE) spectroscopy is a powerful technique for studying paramagnetic species.
  • Electron Nuclear Double Resonance (ENDOR) enhances ESE by probing nuclear spins coupled to electron spins.
  • Optimized microwave cavities are crucial for efficient ENDOR measurements in conjunction with ESE.

Purpose of the Study:

  • To design and characterize a novel microwave cavity for ENDOR experiments.
  • To integrate the cavity with electron spin echo techniques for enhanced sensitivity.
  • To optimize radio-frequency magnetic field generation within the cavity.

Main Methods:

  • Development of a microwave cavity with a target frequency of approximately 9.4 GHz and a Q value of approximately 80.
  • Design modifications to allow for tunable frequency and Q value.
  • Confining the radio-frequency magnetic field to a small volume to minimize inductance and maximize field strength.

Main Results:

  • The developed microwave cavity is suitable for ENDOR measurements coupled with ESE.
  • The cavity's frequency and Q value are adjustable through minor design alterations.
  • Direct measurements indicate a radio-frequency magnetic field of approximately 11 G/A of coil current in the sample, translating to approximately 8 G in the rotating frame per ampere.

Conclusions:

  • The novel microwave cavity design facilitates efficient ENDOR measurements within ESE experiments.
  • The cavity's tunable parameters and strong radio-frequency magnetic field generation offer advantages for spectroscopic studies.
  • This development contributes to improved sensitivity and resolution in electron paramagnetic resonance studies.