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Updated: Jul 2, 2026
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Optimization of data-acquistion rates in time-correlated single-photon fluorimetry
G R Haugen1, B W Wallin, F E Lytle
1General Chemistry Division, Lawrence Livermore Laboratory, University of California, Livermore, California 94550Department of Chemistry, Purdue University, West Lafayette, Indiana 47907.
Abstract:
Four different operating configurations of a time-to-amplitude converter were examined for performance in the time-correlated single-photon fluorimetric experiment. Parameters considered were the intensity/count linearity, maximum data rate, and decay distortion. The inverted configuration with an interactive source was optimum. This arrangement combined total linearity with a 37-kHz conversion rate and did not distort the fluorescence decay. In addition, the pulse-height analyzer could severely limit the overall system data rate and could destroy the TAC intensity/count linearity.
