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Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Updated: Jul 2, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Video fluorometer.

D W Johnson1, J A Gladden, J B Callis

  • 1Department of Chemistry, University of Washington, Seattle, Washington 98195.

The Review of Scientific Instruments
|January 1, 1979
PubMed
Summary
This summary is machine-generated.

A new computer-controlled fluorometer offers simultaneous excitation and emission data acquisition. Its novel optics and sensitive detector enable rapid, broad-range spectral analysis.

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Instrumentation

Background:

  • Fluorometry is a crucial technique for analyzing fluorescent substances.
  • Existing fluorometers often have limitations in spectral range and data acquisition speed.
  • Advancements in detector technology and computer control offer opportunities for improved performance.

Purpose of the Study:

  • To describe a novel computer-controlled multichannel fluorometer.
  • To highlight the unique features of the instrument's optical system and detector.
  • To demonstrate the capability for simultaneous data acquisition over broad spectral ranges.

Main Methods:

  • Development of a computer-controlled multichannel fluorometer.
  • Implementation of a novel optical system for simultaneous excitation and emission measurements.
  • Utilization of a silicon intensified target (SIT) vidicon detector for high sensitivity.
  • Integration with a minicomputer via a video interface for data acquisition.

Main Results:

  • The fluorometer enables simultaneous acquisition of excitation and emission data.
  • Broad spectral ranges can be covered efficiently.
  • High sensitivity is achieved using the SIT vidicon detector.
  • Data acquisition occurs at the standard television rate, allowing for rapid analysis.

Conclusions:

  • The developed fluorometer represents a significant advancement in fluorescence spectroscopy instrumentation.
  • Its unique design allows for efficient and sensitive data collection over wide spectral ranges.
  • The system is well-suited for applications requiring rapid and simultaneous spectral measurements.