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Updated: Jul 2, 2026

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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
Time correlation method for measuring fluorescence decays with a cw laser
C C Dorsey1, M J Pelletier, J M Harris
1Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
The Review of Scientific Instruments
|March 1, 1979
Abstract:
A simple experimental approach to measuring fluorescence decay by time correlation signal analysis is described. The approach allows the use of continuous wave excitation and inexpensive detection-signal processing apparatus. The technique is demonstrated using a cw He-Cd laser. The transient response of the instrument is 0.88 ns, limited by the photomultipliers. The fluorescence lifetime of rose bengal in ethanol is determined, in absence of reorientation relaxation, to be 0.66+/-0.04 ns.
