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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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High resolution cinematography at high temperature in the electron microscope.

The Review of scientific instruments·1970
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Related Experiment Video

Updated: Jul 2, 2026

A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
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Improved grid holders for an all-purpose electron microscope specimen stage.

C E Warble1

  • 1CSIRO Division of Chemical Physics, P.O. Box 160, Clayton, Victoria, Australia 3168.

The Review of Scientific Instruments
|May 1, 1979
PubMed
Summary

Two new specimen grid holders (cups) made from platinum or aluminum offer excellent stability and tilt movements. These designs allow for ultrasonic cleaning and meet stringent demands for all-purpose specimen stages.

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Area of Science:

  • Materials Science
  • Microscopy

Background:

  • Specimen grid holders are crucial components in microscopy stages.
  • Existing holders may not meet the demands of versatile specimen stages.

Purpose of the Study:

  • To discuss general requirements for specimen grid holders.
  • To describe two novel cup models for all-purpose specimen stages.

Main Methods:

  • Design and fabrication of two specimen grid holder models from platinum and aluminum.
  • Evaluation of holder performance concerning stability, tilt, anticontamination, and furnace characteristics.

Main Results:

  • Developed platinum and aluminum cups compatible with all-purpose specimen stages.
  • Achieved excellent stability, tilt movements, anticontamination, and furnace performance.
  • Incorporated ultrasonic cleaning capability for both the cup and grid.

Conclusions:

  • The described specimen grid holders meet stringent requirements for advanced microscopy applications.
  • These novel designs offer enhanced functionality and performance for diverse research needs.