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Updated: Jul 2, 2026
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Published on: August 4, 2009
SERRS-based enzymatic probes for the detection of protease activity
Andrew Ingram1, Louise Byers, Karen Faulds
1Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.
Abstract:
Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be "switched off" and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml-1.
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