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Dietary phytate (inositol hexaphosphate) regulates the activity of intestinal mucosa phytase

E M Onyango1, O Adeola

  • 1Department of Health Sciences, East Tennessee State University, Johnson City, TN 37614, USA. onyango@etsu.edu

Journal of Animal Physiology and Animal Nutrition
|August 15, 2008
PubMed

Insights

Dietary phytate (inositol hexaphosphate) non-competitively inhibits intestinal phytase in chicks, reducing growth and bone ash. Microbial phytase partially restores performance, highlighting phytate

Area of Science:

  • Animal Nutrition
  • Biochemistry
  • Digestive Physiology

Background:

  • Dietary phytate (inositol hexaphosphate) is a major phosphorus source in plant-based animal feeds.
  • Phytate can inhibit the absorption of essential minerals and reduce protein digestibility.
  • Intestinal phytase activity is crucial for phytate hydrolysis and nutrient bioavailability.

Purpose of the Study:

  • To investigate the regulatory role of dietary phytate on intestinal mucosa phytase activity in chicks.
  • To determine the effects of phytate and microbial phytase supplementation on chick growth and bone mineralization.

Main Methods:

  • Chicks were fed purified diets with or without sodium phytate and microbial phytase from 8 to 22 days of age.
  • Duodenal mucosa was analyzed for brush border phytase activity (Vmax and Km).
  • Growth performance (weight gain, feed intake, feed efficiency) and bone ash content were measured.

Main Results:

  • Dietary phytate significantly reduced the Vmax of duodenal brush border phytase without affecting Km, indicating non-competitive inhibition.
  • Phytate supplementation decreased weight gain, feed intake, feed efficiency, and bone ash content.
  • Microbial phytase addition fully restored feed efficiency but only partially improved Vmax and weight gain.

Conclusions:

  • Dietary phytates act as non-competitive inhibitors of intestinal mucosa phytase in chicks.
  • Supplementation with microbial phytase can partially mitigate the negative effects of dietary phytate on growth and nutrient utilization.
  • Understanding phytate-enzyme interactions is vital for optimizing animal nutrition and feed formulation.

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