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Newly identified patterns of Pax2 expression in the developing mouse forebrain.

Vassiliki Fotaki1, David J Price, John O Mason

  • 1Genes and Development Group, Centres for Integrative Physiology and Neuroscience Research, School of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK. vfotaki@ed.ac.uk

BMC Developmental Biology
|August 15, 2008
PubMed
Summary

Pax2 is a useful marker for identifying specific cells in the developing mouse forebrain, particularly within the septum. Its expression pattern aids in understanding septal cell identity and development, even in genetic mutants.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genetics

Background:

  • Transcription factor Pax2 is a known marker in various developing nervous system regions.
  • Previous studies identified Pax2 expression in diencephalic cells forming the eminentia thalami.
  • This prompted a detailed examination of Pax2 in the developing mouse forebrain.

Purpose of the Study:

  • To investigate the expression pattern of Pax2 in the developing mouse forebrain.
  • To evaluate Pax2 as a potential marker for septal cells.
  • To assess Pax2 expression in the context of forebrain developmental mutants.

Main Methods:

  • Analysis of Pax2 protein expression in wild-type mouse embryos from embryonic day (E) 10.5 to postnatal day (P) 15.
  • Examination of Pax2 expression in Pax6Sey/Sey mutant mice with an enlarged septum.
  • Assessment of co-expression with GABAergic and cholinergic markers.

Main Results:

  • Pax2 expression was identified in the septum, hypothalamus, eminentia thalami, and subfornical organ.
  • Pax2 reliably marked septal cells in wild-type and Pax6Sey/Sey mutant mice.
  • These Pax2-expressing septal cells were negative for calbindin and choline acetyltransferase and absent after P15.

Conclusions:

  • Pax2 is expressed in distinct cell populations within the developing septum, hypothalamus, and eminentia thalami.
  • Pax2 serves as a valuable marker for the telencephalic septum due to its early and specific expression.
  • Pax2 expression is maintained in the enlarged septum of Pax6Sey/Sey mutants, confirming its utility.