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Related Concept Videos

Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
Urine Studies I: Urinalysis01:29

Urine Studies I: Urinalysis

Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...
Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
Urologic Endoscopic Procedure: Cystoscopic Examination01:28

Urologic Endoscopic Procedure: Cystoscopic Examination

Meaning of Cystoscopic Examination:Cystoscopy is an essential diagnostic tool in urology that is used to assess the structure and function of the genitourinary system. It provides a direct view of the urethra, bladder, and, in some cases, the ureteral openings. This procedure helps detect structural abnormalities, infections, cancers, and blockages in the urinary tract. There are two types of cystoscopy:Flexible cystoscopy is commonly performed in outpatient settings due to its less invasive...
Imaging Studies V: Intravenous Urography and Retrograde Pyelography01:22

Imaging Studies V: Intravenous Urography and Retrograde Pyelography

IntroductionIntravenous Urography (IVU) and Retrograde Pyelography (RP) are important diagnostic imaging techniques used to evaluate the urinary system. These methods help identify structural abnormalities, obstructions, and functional issues in the kidneys, ureters, and bladder. Both procedures use iodine-based contrast media to enhance the visibility of urinary tract structures on X-ray images, though they differ in their methods and indications.1. Intravenous Urography (IVU)Intravenous...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
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Published on: March 21, 2018

Changes in specimen preparation method may impact urine cytologic evaluation.

Jesse S Voss1, Benjamin R Kipp, Angela K Krueger

  • 1Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, Rochester, MN 55905, USA.

American Journal of Clinical Pathology
|August 15, 2008
PubMed
Summary
This summary is machine-generated.

The ThinPrep technique for urine cytology increased atypical and suspicious diagnoses compared to filtration, but did not alter the detection rate of bladder cancer over one year.

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Published on: December 4, 2020

Area of Science:

  • Urology
  • Cytopathology

Background:

  • Urine cytology is crucial for detecting bladder cancer.
  • Traditional filtration methods have limitations in specimen preparation.
  • The ThinPrep system offers an alternative liquid-based cytology method.

Purpose of the Study:

  • To compare the diagnostic frequency of urine cytology specimens prepared by filtration versus ThinPrep.
  • To evaluate the correlation between cytologic diagnoses and subsequent pathologic findings.
  • To assess the impact of ThinPrep implementation on bladder cancer detection rates.

Main Methods:

  • Retrospective analysis of 2,347 voided urine specimens.
  • Comparison of diagnoses (negative, clusters, positive, atypical, suspicious) before and after ThinPrep implementation.
  • Correlation of cytologic findings with bladder biopsy results for malignancy confirmation.

Main Results:

  • ThinPrep significantly decreased negative diagnoses (85.5% to 78.6%) and increased atypical (3.1% to 8.4%) and suspicious (2.7% to 4.1%) diagnoses.
  • The frequency of positive diagnoses and clusters remained statistically similar between methods.
  • No significant difference in the overall rate of diagnosed bladder carcinoma was observed after one year of follow-up between the two techniques.

Conclusions:

  • The ThinPrep method alters the distribution of urine cytology diagnostic categories, particularly increasing non-negative findings.
  • Despite changes in diagnostic frequencies, ThinPrep did not significantly impact the overall detection rate of bladder carcinoma compared to filtration.
  • Further studies may be warranted to understand the clinical significance of increased atypical and suspicious diagnoses with ThinPrep.