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Updated: Jul 2, 2026
Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
Published on: May 16, 2025
Study of AtSUS2 localization in seeds reveals a strong association with plastids
Juan Gabriel Angeles Núñez1, Jocelyne Kronenberger, Sylvie Wuillème
1INRA, Seed Biology Laboratory, UMR 204, INRA-AgroParisTech, Institut Jean-Pierre Bourgin, INRA, RD10, 78026 Versailles Cedex, France.
Abstract:
Sucrose synthase (SUS) is a key enzyme in sucrose metabolism. This enzyme catalyzes the reversible conversion of sucrose and UDP to UDP-glucose and fructose. In the Arabidopsis SUS gene family (six members), SUS2 is strongly and specifically expressed in Arabidopsis seeds during the maturation phase. Using specific antibodies, we have shown that SUS2 is localized in the embryo, endosperm and seed coat with differential patterns. During the maturation phase, the SUS2 protein seems to be mainly co-localized with plastids in the embryo. This novel finding is discussed in relation to the role of this enzyme in storage organs.
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