Clinically relevant advances in on-chip affinity-based electrophoresis and electrochromatography

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Clinical Diagnostics

Background:

• Clinical diagnostics are crucial for personalized medicine, global health, and continuous health monitoring.
• Lab-on-a-chip (LOC) technology offers performance advantages for analytical techniques.
• Affinity-based electrokinetic separations are a key area for LOC development.

Purpose of the Study:

• To review recent advancements in LOC diagnostic devices utilizing affinity-based electrokinetic separations.
• To highlight the integration of sample preparation functions within LOC systems.
• To explore the potential for multiplexed analyses and near-patient diagnostic applications.

Main Methods:

• Survey of current literature on LOC technologies for affinity-based electrokinetic separations.
• Analysis of device designs for protein quantitation and sample preparation.
• Examination of emerging multiplexing capabilities in diagnostic assays.

Main Results:

• LOC platforms demonstrate significant potential for protein quantitation using affinity-based electrokinetic separations.
• Integration of preparatory steps streamlines complex biological sample analysis.
• Initial developments show promise for multiplexed assays and point-of-care applications.

Conclusions:

• LOC-based affinity electrokinetic separations are advancing clinical and point-of-care diagnostics.
• These technologies facilitate the translation of laboratory assays to near-patient settings.
• The reviewed developments support personalized medicine and improved global health strategies.